Journal: Scientific reports

Article Title: Disordered metabolism in mice lacking irisin.

doi: 10.1038/s41598-020-74588-7

Figure Lengend Snippet: Figure 6. Increased bone resorption in irisin lacking mice. (A, B) Sections of the distal shaft of the femur were stained with osteoprotegerin (OPG) (1:60) and receptor activator of nuclear factor-kB ligand (RANKL) (1:80) antibodies. Positive (black arrows) osteoblasts are shown, 200×. (C) Representative images of the distal metaphyseal region of the femur, together with cell counts (osteoclasts) per bone perimeter (Bpm). Red arrows, tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts, 100×, (NIS-Elements Viewer, v4.2.0; https:// www.downza.cn/soft/2751-21.html); (D) serum levels of osteocalcin TRAP (with three replicates). (Graphpad Prism, v7.0, https://www.xue51.com/soft/3932.html). Data are presented as the mean ± SEM (n = 15 per group). *P < 0.05, **P < 0.01 compared to the WT group.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) kit for osteocalcin (OCN) (E06917m), Alkaline Phosphatase (ALP) (E0200m), and Tartrate-resistant acid phosphatase (TRAP) (E08492m) were purchased from Elabscience (Wuhan, China).

Techniques: Staining

Journal: Scientific Reports

Article Title: Neuronal TRPV1 activation regulates alveolar bone resorption by suppressing osteoclastogenesis via CGRP

doi: 10.1038/srep29294

Figure Lengend Snippet: ( a ) Raw 264.7 cells were treated with RANKL (100 ng/mL)/ P. gingivalis LPS (1 μg/mL) and cultured for 5 days with the indicated concentrations of recombinant CGRP. Representative images of TRAP staining at low magnification (upper panels) and high magnification (lower panels). Scale bars represent 500 μm. ( b ) The numbers (left) and the areas (right) of TRAP-positive multinucleated (≥3 nuclei per cells) cells (MNCs) (n = 5 in each group). All data are mean ± SD (*p < 0.05 and **p < 0.01 versus 0 nM CGRP or as indicated, by ANOVA). ( c ) Low magnification images (upper panels) and high magnification images (lower panels) of a bone resorption pit assay. Scale bars represent 400 μm.( d ) Percentage of bone surface area resorbed (n = 5 in each group). All data are mean ± SD (*p < 0.05 and **p < 0.01 versus 0 nM CGRP, by ANOVA).

Article Snippet: For morphological analysis, the cells were stained using a TRAP staining kit (Primary Cell Co., Ltd., Sapporo, Japan).

Techniques: Cell Culture, Recombinant, Staining

Journal: Scientific Reports

Article Title: Neuronal TRPV1 activation regulates alveolar bone resorption by suppressing osteoclastogenesis via CGRP

doi: 10.1038/srep29294

Figure Lengend Snippet: ( a ) Daily administration of capsaicin suppressed alveolar bone resorption in ligated mice. The normal diet group was fed standard laboratory chow, the other group was fed standard laboratory chow plus 0.01% (100 ppm) capsaicin for 14 days. On day 7, half of the mice from each group underwent ligation, and all mice were sacrificed for analysis on day 14. ( b ) The CEJ–ABC distance was significantly lower in ligated-mice fed with capsaicin compared to mice fed with the normal diet (n = 6 in each group) ( c ) Representative TRAP staining images in interradicular septa. Scale bars represent 200 μm. ( d ) Measurement of TRAP-positive areas adjacent to the bone surface in interradicular septa regions (n = 3 in each group). All data are mean ± SD (*p < 0.05 and **p < 0.01 versus unligated mice with normal diet or as indicated, by ANOVA). ( e ) Quantification of alveolar bone loss in Trpv1 −/− and ( f ) RTX-treated mice (n = 6 in each group). All data are mean ± SD (**p < 0.01 versus unligated mice with normal diet, by ANOVA).

Article Snippet: For morphological analysis, the cells were stained using a TRAP staining kit (Primary Cell Co., Ltd., Sapporo, Japan).

Techniques: Ligation, Staining

Journal: Scientific Reports

Article Title: Neuronal TRPV1 activation regulates alveolar bone resorption by suppressing osteoclastogenesis via CGRP

doi: 10.1038/srep29294

Figure Lengend Snippet: ( a ) Immunofluorescent staining for CGRP and PGP9.5, a neuronal maker (left panel) and TRAP staining (right panel) in consecutive tissue sections of gingival tissues. The arrowhead indicates CGRP-containing nerve fibers that are in the vicinity of osteoclasts on the alveolar bone surface. OC, osteoclasts. AB, alveolar bone. D, dentin. Scale bars represent 50 μm. ( b ) Model of the relative roles of TRPV1 and CGRP on bone remodeling in periodontitis. Activation of TRPV1 on sensory neurons innervating gingival tissues induces afferent input to TG, which results in the synthesis of neuropeptides such as CGRP. Anterograde axonal transportation of CGRP to peripheral tissue and subsequent release of CGRP leads to the inhibition of osteoclast differentiation in alveolar bone.

Article Snippet: For morphological analysis, the cells were stained using a TRAP staining kit (Primary Cell Co., Ltd., Sapporo, Japan).

Techniques: Staining, Activation Assay, Inhibition

Journal: Biomedical Reports

Article Title: Effects of aged garlic extract on experimental periodontitis in mice

doi: 10.3892/br.2025.1975

Figure Lengend Snippet: Histological analysis of maxillae and surrounding periodontal tissues in mice with ligature-induced experimental periodontitis. (A and B) Hematoxylin and eosin staining. The enlarged images within the red circles in A are shown in B. (C and D) TRAP staining. The enlarged images within the red circles in C are shown in D. Representative sections for each condition (control group: n=5, AGE group: n=6) are shown using serial sections. Red arrow: osteoclasts. Scale bar, 100 µm. (E) The mean and standard deviations of the relative numbers of TRAP-positive cells on the un-ligated and ligated sides of each mouse. After analysis using one-way ANOVA followed by Tukey's multiple-comparison test, a two-tailed Student's t-test was used for two-group comparisons. * P<0.05. TRAP, tartrate-resistant acid phosphatase; AGE, aged garlic extract.

Article Snippet: Osteoclasts were visualized using a tartrate-resistant acid phosphatase (TRAP) staining kit (FUJIFILM Wako Pure Chemical Corporation).

Techniques: Staining, Control, Comparison, Two Tailed Test